|
|
| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
Registro completo
|
Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
29/10/2020 |
Actualizado : |
27/01/2021 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
GIANNITTI, F.; GARCÍA, J.P.; ROOD, J.I.; ADAMS, V.; ARMENDANO, J.I.; BEINGESSER, J.; UZAL, F.A. |
Afiliación : |
FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JORGE P. GARCÍA, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), Tandil, Buenos Aires, Argentina.; JULIAN I. ROOD, Monash University, Clayton, Victoria, Australia.; VICKI ADAMS, Monash University, Clayton, Victoria, Australia; JOAQUÍN I. ARMENDANO, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), Tandil, Buenos Aires, Argentina.; JULIANN BEINGESSER, University of California at Davis, San Bernardino, CA, USA.; FRANCISCO A. UZAL, University of California at Davis, San Bernardino, CA, USA. |
Título : |
Cardiopulmonary Lesions in Sheep Produced by Experimental Acute Clostridium Perfringens Type D Enterotoxemia. |
Fecha de publicación : |
2021 |
Fuente / Imprenta : |
Veterinay Pathology, volumen 58, number 1, pag. 103-113, 2021. Doi: https://doi.org/10.1177/0300985820965554 |
DOI : |
10.1177/0300985820965554 |
Idioma : |
Inglés |
Notas : |
Article history: First Published October 15, 2020. Corresponding Author:Francisco A. Uzal, Email: fuzal@cahfs.ucdavis.edu. The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: Federico Giannitti acknowledges support from the Uruguayan ?Agencia Nacional de Investigaci ´on e Innovaci ´on? (ANII) through mobility grant MOV_CA_2018_1_150021. This work was supported by Grant R01 AI056177 from the National Institute of Allergy and Infectious Diseases (NIAID). Research at Monash University was also supported by funding provided by the Australian Research Council to the Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics (Grant CE0562063). |
Contenido : |
Abstract:Enterotoxemia caused by Clostridium perfringens type D is one of the most prevalent clostridial diseases of sheep. The lesions of
the acute form of this disease, particularly the cerebral lesions, are well characterized; however, detailed descriptions of the cardiac and pulmonary lesions are lacking. Here we describe cardiopulmonary lesions in experimental acute type D enterotoxemia in sheep and determine the role of epsilon toxin (ETX) in the development of these lesions. Four groups of 6 sheep were intraduodenally inoculated with either a wild-type C. perfringens type D strain; its etx knockout mutant, which is unable to produce ETX; the etx mutant complemented with the wild-type etx gene, which regains the ETX toxigenic ability; or sterile culture
medium as a control. All sheep were subjected to postmortem examination within 24 hours of inoculation. Lesion scores were compared between groups for pulmonary edema; hydrothorax; ascites; hydropericardium; endocardial, myocardial and epicardial hemorrhages; microscopic lesions of acute myocardial degeneration and necrosis; and myocardial, endocardial, and epicardial edema, hemorrhage, and inflammation. Only sheep inoculated with the wild-type and complemented ETX-toxigenic bacterial strains developed cardiopulmonary lesions, which were present in varying degrees of severity and proportions. These lesions were not present in sheep inoculated with the etx mutant or in the negative control. We conclude that severe acute cardiopulmonary lesions in sheep with experimental enterotoxemia are associated with the capacity of the strains to produce ETX. These changes are likely contributors to the clinical signs and even death of affected animals. MenosAbstract:Enterotoxemia caused by Clostridium perfringens type D is one of the most prevalent clostridial diseases of sheep. The lesions of
the acute form of this disease, particularly the cerebral lesions, are well characterized; however, detailed descriptions of the cardiac and pulmonary lesions are lacking. Here we describe cardiopulmonary lesions in experimental acute type D enterotoxemia in sheep and determine the role of epsilon toxin (ETX) in the development of these lesions. Four groups of 6 sheep were intraduodenally inoculated with either a wild-type C. perfringens type D strain; its etx knockout mutant, which is unable to produce ETX; the etx mutant complemented with the wild-type etx gene, which regains the ETX toxigenic ability; or sterile culture
medium as a control. All sheep were subjected to postmortem examination within 24 hours of inoculation. Lesion scores were compared between groups for pulmonary edema; hydrothorax; ascites; hydropericardium; endocardial, myocardial and epicardial hemorrhages; microscopic lesions of acute myocardial degeneration and necrosis; and myocardial, endocardial, and epicardial edema, hemorrhage, and inflammation. Only sheep inoculated with the wild-type and complemented ETX-toxigenic bacterial strains developed cardiopulmonary lesions, which were present in varying degrees of severity and proportions. These lesions were not present in sheep inoculated with the etx mutant or in the negative control. We conclude that severe acute ... Presentar Todo |
Palabras claves : |
CLOSTRIDIUM PERFRINGENS TYPE D; EPSILON TOXIN; HEART; LUNGS; PATHOLOGY; PLATAFORMA SALUD ANIMAL; SHEEP. |
Thesagro : |
ENFERMEDADES DE LOS ANIMALES; OVEJA. |
Asunto categoría : |
-- |
Marc : |
LEADER 03466naa a2200325 a 4500 001 1061439 005 2021-01-27 008 2021 bl uuuu u00u1 u #d 024 7 $a10.1177/0300985820965554$2DOI 100 1 $aGIANNITTI, F. 245 $aCardiopulmonary Lesions in Sheep Produced by Experimental Acute Clostridium Perfringens Type D Enterotoxemia.$h[electronic resource] 260 $c2021 500 $aArticle history: First Published October 15, 2020. Corresponding Author:Francisco A. Uzal, Email: fuzal@cahfs.ucdavis.edu. The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: Federico Giannitti acknowledges support from the Uruguayan ?Agencia Nacional de Investigaci ´on e Innovaci ´on? (ANII) through mobility grant MOV_CA_2018_1_150021. This work was supported by Grant R01 AI056177 from the National Institute of Allergy and Infectious Diseases (NIAID). Research at Monash University was also supported by funding provided by the Australian Research Council to the Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics (Grant CE0562063). 520 $aAbstract:Enterotoxemia caused by Clostridium perfringens type D is one of the most prevalent clostridial diseases of sheep. The lesions of the acute form of this disease, particularly the cerebral lesions, are well characterized; however, detailed descriptions of the cardiac and pulmonary lesions are lacking. Here we describe cardiopulmonary lesions in experimental acute type D enterotoxemia in sheep and determine the role of epsilon toxin (ETX) in the development of these lesions. Four groups of 6 sheep were intraduodenally inoculated with either a wild-type C. perfringens type D strain; its etx knockout mutant, which is unable to produce ETX; the etx mutant complemented with the wild-type etx gene, which regains the ETX toxigenic ability; or sterile culture medium as a control. All sheep were subjected to postmortem examination within 24 hours of inoculation. Lesion scores were compared between groups for pulmonary edema; hydrothorax; ascites; hydropericardium; endocardial, myocardial and epicardial hemorrhages; microscopic lesions of acute myocardial degeneration and necrosis; and myocardial, endocardial, and epicardial edema, hemorrhage, and inflammation. Only sheep inoculated with the wild-type and complemented ETX-toxigenic bacterial strains developed cardiopulmonary lesions, which were present in varying degrees of severity and proportions. These lesions were not present in sheep inoculated with the etx mutant or in the negative control. We conclude that severe acute cardiopulmonary lesions in sheep with experimental enterotoxemia are associated with the capacity of the strains to produce ETX. These changes are likely contributors to the clinical signs and even death of affected animals. 650 $aENFERMEDADES DE LOS ANIMALES 650 $aOVEJA 653 $aCLOSTRIDIUM PERFRINGENS TYPE D 653 $aEPSILON TOXIN 653 $aHEART 653 $aLUNGS 653 $aPATHOLOGY 653 $aPLATAFORMA SALUD ANIMAL 653 $aSHEEP 700 1 $aGARCÍA, J.P. 700 1 $aROOD, J.I. 700 1 $aADAMS, V. 700 1 $aARMENDANO, J.I. 700 1 $aBEINGESSER, J. 700 1 $aUZAL, F.A. 773 $tVeterinay Pathology, volumen 58, number 1, pag. 103-113, 2021. Doi: https://doi.org/10.1177/0300985820965554
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA La Estanzuela (LE) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
Registro completo
|
Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
26/03/2022 |
Actualizado : |
26/03/2022 |
Tipo de producción científica : |
Abstracts/Resúmenes |
Autor : |
CIUFFO, C.; ARÉVALO, A.P.; GARCIA, F.; PERDOMO, T.; CRISPO, M.; GIANNITTI, F.; BUSCHIAZZO, A.; ZARANTONELLI, L. |
Afiliación : |
CAMILA CIUFFO, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; ANA PAULA ARÉVALO, Unidad de Biotecnología en Animales de Laboratorio, Institut Pasteur de Montevideo, Montevideo, Uruguay.; FEDERICO GARCIA SUAREZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; TERESITA YISELL PERDOMO TORRES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARTINA CRISPO, Unidad de Biotecnología en Animales de Laboratorio, Institut Pasteur de Montevideo, Montevideo, Uruguay.; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALEJANDRO BUSCHIAZZO, Laboratorio de Microbiología Molecular y Estructural, Institut Pasteur de Montevideo, Montevideo, Uruguay.; LETICIA ZARANTONELLI, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; Laboratorio de Microbiología Molecular y Estructural, Institut Pasteur de Montevideo, Montevideo, Uruguay. |
Título : |
Estudio de la capacidad infectiva, colonización renal e inmunogenicidad de Leptospira interrogans serogrupo Pomona serovar Kennewicki en un modelo murino sub-letal y crónico. [abstract]. |
Complemento del título : |
Sub-área 4: Patogenia, virulencia y resistencia. |
Fecha de publicación : |
2021 |
Fuente / Imprenta : |
In: Anais III Consensos Latinoamericanos em Leptospirose Animal. Nov 30-Dic 02, 2021 (evento online). |
Idioma : |
Español |
Contenido : |
En Uruguay, Leptospira interrogans sg Pomona sv Kennewicki es la variante más frecuentemente aislada en bovinos. También ha sido identificada en brotes de leptospirosis en ovinos y en jabalíes (Sus scrofa), así como en humanos, lo cual sugiere fuertemente la transmisión zoonótica. El objetivo de este estudio fue evaluar atributos de virulencia de L. interrogans sg Pomona sv Kennewicki en un modelo murino, mimetizando una vía de infección natural. |
Palabras claves : |
Leptospira interrogans; PLATAFORMA DE SALUD ANIMAL. |
Thesagro : |
LEPTOSPIROSIS. |
Asunto categoría : |
L73 Enfermedades de los animales |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16360/1/Estudio-de-la-capacidad-infectiva-colonizacion-renal-e-inmunogenicidad-de-Leptospira-interrogans-abstract-2021.pdf
https://www.faculdadeinove.me/3clla/edicoes/iiiclla/anais
|
Marc : |
LEADER 01295nam a2200229 a 4500 001 1062919 005 2022-03-26 008 2021 bl uuuu u01u1 u #d 100 1 $aCIUFFO, C. 245 $aEstudio de la capacidad infectiva, colonización renal e inmunogenicidad de Leptospira interrogans serogrupo Pomona serovar Kennewicki en un modelo murino sub-letal y crónico. [abstract].$h[electronic resource] 260 $aIn: Anais III Consensos Latinoamericanos em Leptospirose Animal. Nov 30-Dic 02, 2021 (evento online).$c2021 520 $aEn Uruguay, Leptospira interrogans sg Pomona sv Kennewicki es la variante más frecuentemente aislada en bovinos. También ha sido identificada en brotes de leptospirosis en ovinos y en jabalíes (Sus scrofa), así como en humanos, lo cual sugiere fuertemente la transmisión zoonótica. El objetivo de este estudio fue evaluar atributos de virulencia de L. interrogans sg Pomona sv Kennewicki en un modelo murino, mimetizando una vía de infección natural. 650 $aLEPTOSPIROSIS 653 $aLeptospira interrogans 653 $aPLATAFORMA DE SALUD ANIMAL 700 1 $aARÉVALO, A.P. 700 1 $aGARCIA, F. 700 1 $aPERDOMO, T. 700 1 $aCRISPO, M. 700 1 $aGIANNITTI, F. 700 1 $aBUSCHIAZZO, A. 700 1 $aZARANTONELLI, L.
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA Las Brujas (LB) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
Expresión de búsqueda válido. Check! |
|
|